Kinetic behaviors of Inulin hydrolysis by inulinase from Kluyveromyces
نویسندگان
چکیده
Enzyme hydrolysis kinetic model of inulin by inulinase from Kluyveromyces lactis was established. The corresponding Michaelis-Menten equation model constants were found to be related to temperature through the Arrhenius model. The kinetic model was validated by experimental data and also be utilized to predict the hydrolysis reaction rates of the inulin for substrate concentrations ranging between 2 and 200 g/L and a fairly large reaction temperature range (35-55 oC). Furthermore, this kinetic model can provide a scientific and convenient way to choose the optimized hydrolysis conditions for the production of fermentable sugar for ethanol production.
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